Casein Kinase 1 gamma 2 Kit ELISA

N° du produit ABIN6951105

Détail du produit Casein Kinase 1 gamma 2 Kit ELISA

Antigène: Casein Kinase 1 gamma 2 (CSNK1G2) (Casein Kinase 1, gamma 2 (CSNK1G2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human CSNK1G2 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: Casein Kinase 1 gamma 2 (CSNK1G2) (Casein Kinase 1, gamma 2 (CSNK1G2))

Autre désignation: CSNK1G2 (CSNK1G2 Produits)

Synonymes: CK1g2 Kit ELISA, 2810429I12Rik Kit ELISA, AI463719 Kit ELISA, CKI Kit ELISA, ck1-gamma Kit ELISA, CSNK1G2 Kit ELISA, ck1g2 Kit ELISA, csnk1g2 Kit ELISA, fj97g11 Kit ELISA, wu:fj97g11 Kit ELISA, wz8345 Kit ELISA, zgc:110719 Kit ELISA, zgc:153415 Kit ELISA, casein kinase 1 gamma 2 Kit ELISA, casein kinase 1, gamma 2 Kit ELISA, casein kinase 1 gamma 2 L homeolog Kit ELISA, casein kinase 1, gamma 2a Kit ELISA, casein kinase 1, gamma 2b Kit ELISA, CSNK1G2 Kit ELISA, Csnk1g2 Kit ELISA, csnk1g2.L Kit ELISA, csnk1g2 Kit ELISA, csnk1g2a Kit ELISA, csnk1g2b Kit ELISA

ID gène: 1455

UniProt: P78368

Pathways: Signalisation Hedgehog