Coagulation Factor IX Kit ELISA

N° du produit ABIN6951106

Détail du produit Coagulation Factor IX Kit ELISA

Antigène: Coagulation Factor IX (F9)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human F9 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: Coagulation Factor IX (F9)

Autre désignation: F9 (F9 Produits)

Synonymes: AA407498 Kit ELISA, AI506714 Kit ELISA, AK-3 Kit ELISA, Ak3l Kit ELISA, Ak3l1 Kit ELISA, Akl3l Kit ELISA, FIX Kit ELISA, HEMB Kit ELISA, P19 Kit ELISA, PTC Kit ELISA, THPH8 Kit ELISA, AW111646 Kit ELISA, Cf-9 Kit ELISA, Cf9 Kit ELISA, f9 Kit ELISA, zgc:109775 Kit ELISA, fix Kit ELISA, ptc Kit ELISA, hemb Kit ELISA, FIXA Kit ELISA, zgc:136807 Kit ELISA, adenylate kinase 3 Kit ELISA, coagulation factor IX Kit ELISA, coagulation factor IXa Kit ELISA, coagulation factor 9 Kit ELISA, coagulation factor 9 S homeolog Kit ELISA, Coagulation factor IX Kit ELISA, coagulation factor IXb Kit ELISA, Ak3 Kit ELISA, F9 Kit ELISA, f9a Kit ELISA, f9 Kit ELISA, CpipJ_CPIJ003776 Kit ELISA, CpipJ_CPIJ009129 Kit ELISA, CpipJ_CPIJ013063 Kit ELISA, CpipJ_CPIJ013624 Kit ELISA, Tsp_01441 Kit ELISA, f9.S Kit ELISA, fa9 Kit ELISA, f9b Kit ELISA

ID gène: 2158

UniProt: P00740