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Ephrin B3 Kit ELISA

EFNB3 Reactivité: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951118
  • Antigène Voir toutes Ephrin B3 (EFNB3) Kits ELISA
    Ephrin B3 (EFNB3)
    Reactivité
    • 3
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Rat Ephrin-B3 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product EFNB3 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Voir toutes Ephrin B3 (EFNB3) Kits ELISA
    Ephrin B3 (EFNB3)
    Autre désignation
    Ephrin-B3 (EFNB3 Produits)
    Synonymes
    efnb3-A Kit ELISA, EphB3 Kit ELISA, EFNB3 Kit ELISA, efnb3 Kit ELISA, id:ibd5049 Kit ELISA, zgc:111938 Kit ELISA, EFL-6 Kit ELISA, ELF-3 Kit ELISA, Elk-L3 Kit ELISA, Epl8 Kit ELISA, LERK-8 Kit ELISA, NLERK-2 Kit ELISA, ELK-L3 Kit ELISA, RGD1565678 Kit ELISA, EFL6 Kit ELISA, EPLG8 Kit ELISA, LERK8 Kit ELISA, ephrin B3 Kit ELISA, ephrin-B3 L homeolog Kit ELISA, ephrin-B3b Kit ELISA, ephrin-B3 Kit ELISA, EFNB3 Kit ELISA, efnb3.L Kit ELISA, efnb3b Kit ELISA, efnb3 Kit ELISA, Efnb3 Kit ELISA
    ID gène
    360546
    UniProt
    G3V7D4
    Pathways
    Signalisation RTK
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