FKBP1A Kit ELISA

N° du produit ABIN6951130

Détail du produit FKBP1A Kit ELISA

Antigène: FKBP1A (FK506 Binding Protein 1A, 12kDa (FKBP1A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human FKBP12 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: FKBP1A (FK506 Binding Protein 1A, 12kDa (FKBP1A))

Autre désignation: FKBP12 (FKBP1A Produits)

Synonymes: 12kDa Kit ELISA, FKBP12 Kit ELISA, FKBP12-T1 Kit ELISA, FKBP12-T2 Kit ELISA, Fkbp Kit ELISA, Fkbp1 Kit ELISA, mFKBP1 Kit ELISA, mFKBP12 Kit ELISA, FKBP-12 Kit ELISA, FKBP-1A Kit ELISA, FKBP1 Kit ELISA, Rotamase Kit ELISA, Fkbp12 Kit ELISA, ARABIDOPSIS THALIANA FK506-BINDING PROTEIN 12 Kit ELISA, ATFKBP12 Kit ELISA, FK506-binding protein 12 Kit ELISA, IMMUNOPHILIN Kit ELISA, MSJ1.19 Kit ELISA, MSJ1_19 Kit ELISA, PKC12 Kit ELISA, PKCI2 Kit ELISA, PPIASE Kit ELISA, FKBP1B Kit ELISA, Fkbp2 Kit ELISA, fj02g10 Kit ELISA, zgc:73373 Kit ELISA, wu:fj02g10 Kit ELISA, FKBP1A Kit ELISA, Bmfkbp13 Kit ELISA, zgc:103752 Kit ELISA, FK506 binding protein 1a Kit ELISA, FK506 binding protein 1A Kit ELISA, FK506-binding protein Kit ELISA, FK506-binding protein 12 Kit ELISA, FK506 binding protein 1Aa Kit ELISA, FK506 binding protein 1C Kit ELISA, FK506-binding protein 59 Kit ELISA, FK506 binding protein like Kit ELISA, FK506 binding protein 1A L homeolog Kit ELISA, FK506 binding protein 1Ab Kit ELISA, Fkbp1a Kit ELISA, FKBP1A Kit ELISA, Fkbp12 Kit ELISA, FKBP12 Kit ELISA, fkbp1aa Kit ELISA, FKBP1C Kit ELISA, EHI_180160 Kit ELISA, LOC5573443 Kit ELISA, Fkbp13 Kit ELISA, FKBPL Kit ELISA, fkbp1a.L Kit ELISA, fkbp1ab Kit ELISA

ID gène: 2280

UniProt: P62942

Pathways: Negative Regulation of Transporter Activity, Methionine Biosynthetic Process