Glutaredoxin 1 Kit ELISA

N° du produit ABIN6951137

Détail du produit Glutaredoxin 1 Kit ELISA

Antigène: Glutaredoxin 1 (GRX1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human GLRX ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: Glutaredoxin 1 (GRX1)

Autre désignation: GLRX (GRX1 Produits)

Synonymes: Glrx1 Kit ELISA, Grx Kit ELISA, grx Kit ELISA, wu:fc38f02 Kit ELISA, zgc:103707 Kit ELISA, GLRXL Kit ELISA, Grx1 Kit ELISA, TTase Kit ELISA, C86710 Kit ELISA, D13Wsu156e Kit ELISA, grx1 Kit ELISA, GLRX Kit ELISA, GRX Kit ELISA, GRX1 Kit ELISA, GLRX1 Kit ELISA, TTF Kit ELISA, glrx Kit ELISA, glutaredoxin Kit ELISA, glutaredoxin (thioltransferase) Kit ELISA, glutaredoxin Grx1 Kit ELISA, NrdH-redoxin Kit ELISA, Uncharacterized monothiol glutaredoxin F10D7.3 Kit ELISA, glutaredoxin-1 (Grx1) Kit ELISA, temporal expression: late Kit ELISA, glutaredoxin L homeolog Kit ELISA, Glrx Kit ELISA, glrx Kit ELISA, GLRX Kit ELISA, grx1 Kit ELISA, AF_RS07750 Kit ELISA, F10D7.3 Kit ELISA, AFUA_1G06100 Kit ELISA, NT01EI_2528 Kit ELISA, O2L Kit ELISA, glrx.L Kit ELISA

Classe de substances: Viral Protein

ID gène: 2745

UniProt: P35754

Pathways: Cell RedoxHomeostasis