ING5 Kit ELISA

N° du produit ABIN6951148

Détail du produit ING5 Kit ELISA

Antigène: ING5 (Inhibitor of Growth Family, Member 5 (ING5))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human ING5 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: ING5 (Inhibitor of Growth Family, Member 5 (ING5))

Autre désignation: ING5 (ING5 Produits)

Synonymes: p28ING5 Kit ELISA, 1700001C14Rik Kit ELISA, 1700027H23Rik Kit ELISA, 1810018M11Rik Kit ELISA, AI225768 Kit ELISA, p28ing5 Kit ELISA, MGC76007 Kit ELISA, ING5 Kit ELISA, MGC133862 Kit ELISA, ING1 Kit ELISA, p33 Kit ELISA, si:busm1-40g1.1 Kit ELISA, si:dz40g1.1 Kit ELISA, wu:fc94a02 Kit ELISA, zgc:100910 Kit ELISA, ing5 Kit ELISA, inhibitor of growth family member 5 Kit ELISA, inhibitor of growth family, member 5 Kit ELISA, inhibitor of growth family member 5 L homeolog Kit ELISA, inhibitor of growth family, member 5b Kit ELISA, inhibitor of growth family, member 5a Kit ELISA, ING5 Kit ELISA, Ing5 Kit ELISA, ing5 Kit ELISA, ing5.L Kit ELISA, ing5b Kit ELISA, ing5a Kit ELISA

ID gène: 84289

UniProt: Q8WYH8

Pathways: Chromatin Binding