MAX Kit ELISA

N° du produit ABIN6951174

Détail du produit MAX Kit ELISA

Antigène: MAX (MYC Associated Factor X (MAX))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human MAX ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: MAX (MYC Associated Factor X (MAX))

Autre désignation: MAX (MAX Produits)

Synonymes: CG9648 Kit ELISA, Dmel\\CG9648 Kit ELISA, MAX Kit ELISA, bHLHd9 Kit ELISA, dMax Kit ELISA, dmax Kit ELISA, max Kit ELISA, bhlhd4 Kit ELISA, bhlhd5 Kit ELISA, bhlhd6 Kit ELISA, bhlhd7 Kit ELISA, bhlhd8 Kit ELISA, xmax2 Kit ELISA, xmax4 Kit ELISA, xmax Kit ELISA, AA960152 Kit ELISA, AI875693 Kit ELISA, bHLHd4 Kit ELISA, bHLHd5 Kit ELISA, bHLHd6 Kit ELISA, bHLHd7 Kit ELISA, bHLHd8 Kit ELISA, fa92b04 Kit ELISA, fb52a04 Kit ELISA, fb95h11 Kit ELISA, wu:fa92b04 Kit ELISA, wu:fb52a04 Kit ELISA, wu:fb95h11 Kit ELISA, zmax Kit ELISA, CG9648 gene product from transcript CG9648-RD Kit ELISA, MYC associated factor X L homeolog Kit ELISA, MYC associated factor X Kit ELISA, Max protein Kit ELISA, MYC associated factor X S homeolog Kit ELISA, myc associated factor X Kit ELISA, Max Kit ELISA, max.L Kit ELISA, MAX Kit ELISA, max Kit ELISA, max.S Kit ELISA

ID gène: 4149

UniProt: P61244

Pathways: Mitotic G1-G1/S Phases