TRKA Kit ELISA
NTRK1
Reactivité: Rat
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951191
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Antigène
Voir toutes TRKA (NTRK1) Kits ELISA
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TRKA (NTRK1)
(Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))
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Reactivité
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Rat
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Méthode de détection
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Colorimetric
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Type de méthode
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Sandwich ELISA
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Application
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ELISA
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Fonction
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Rat TrkA ELISA Kit.
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Type d'échantillon
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Indications d'application
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Optimal working dilution should be determined by the investigator.
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Plaque
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Pre-coated
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Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Date de péremption
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6 months
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Antigène
Voir toutes TRKA (NTRK1) Kits ELISA
-
TRKA (NTRK1)
(Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))
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Autre désignation
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TrkA (NTRK1 Produits)
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Synonymes
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MTC Kit ELISA, TRK Kit ELISA, TRK1 Kit ELISA, TRKA Kit ELISA, Trk-A Kit ELISA, p140-TrkA Kit ELISA, CDHF12 Kit ELISA, CDHR16 Kit ELISA, HSCR1 Kit ELISA, MEN2A Kit ELISA, MEN2B Kit ELISA, MTC1 Kit ELISA, PTC Kit ELISA, RET-ELE1 Kit ELISA, RET51 Kit ELISA, Trk Kit ELISA, C80751 Kit ELISA, Tkr Kit ELISA, TrkA Kit ELISA, trk Kit ELISA, trka Kit ELISA, CTRKA Kit ELISA, trkA Kit ELISA, neurotrophic receptor tyrosine kinase 1 Kit ELISA, ret proto-oncogene Kit ELISA, neurotrophic tyrosine kinase, receptor, type 1 Kit ELISA, NTRK1 Kit ELISA, RET Kit ELISA, Ntrk1 Kit ELISA, ntrk1 Kit ELISA
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ID gène
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59109
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UniProt
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P35739
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Pathways
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Signalisation RTK, Neurotrophin Signaling Pathway, cAMP Metabolic Process
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