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RPS6KB1 Kit ELISA
RPS6KB1
Reactivité: Humain
pSer424
Colorimetric
Sandwich ELISA
Cell Lysate, Tissue Lysate
N° du produit ABIN6951204
Détail du produit RPS6KB1 Kit ELISA
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Antigène
Voir toutes RPS6KB1 Kits ELISA
RPS6KB1
(Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))
Épitope
pSer424, pThr421
Reactivité
Toutes les réactivités sur RPS6KB1 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Human Phospho-P70S6K (T421/S424) Quantitative ELISA Kit. This assay quantitatively measures phosphorylated P70S6K (Thr421/S424) in lysate samples.
Type d'échantillon
Cell Lysate, Tissue Lysate
Analytical Method
Quantitative
Specificité
The antibody pair provided in this kit recognizes human p70S6 Kinase phosphorylated at sites Threonine-421 and/or Serine-424.
Attributs du produit
Rapidly measure phosphorylated protein in lysates Screen numerous different cell lysates without performing a Western Blot analysis Minimal hands-on time, convenient, and non-radioactive material
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Anti-Phospho Antibody HRP-Conjugated Secondary Antibody Assay Diluent TMB One-Step Substrate Stop Solution Lysis Buffer Positive Control Sample
Matériel non inclus
Distilled or deionized water 100 mL and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors Precision pipettes to deliver 2 μL to 1 mL volumes Adjustable 1-25 mL pipettes for reagent preparation Benchtop rocker or shaker Microplate reader capable of measuring absorbance at 450 nm
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Plaque
Pre-coated
Protocole
Prepare all reagents and samples as instructed in the manual. Add 100 μL of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared 1X HRP-Streptavidin to each well. Incubate 1 h at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes RPS6KB1 Kits ELISA
RPS6KB1
(Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))
Autre désignation
P70S6K (RPS6KB1 Produits )
Synonymes
PS6K Kit ELISA, S6K Kit ELISA, S6K-beta-1 Kit ELISA, S6K1 Kit ELISA, STK14A Kit ELISA, p70 S6KA Kit ELISA, p70(S6K)-alpha Kit ELISA, p70-S6K Kit ELISA, p70-alpha Kit ELISA, 10539 Kit ELISA, 7084 Kit ELISA, CG10539 Kit ELISA, DS6K Kit ELISA, Dmel\\CG10539 Kit ELISA, Dp70S6k Kit ELISA, Dp70[s6k] Kit ELISA, Dp70s6k Kit ELISA, S6k Kit ELISA, dS6K Kit ELISA, dS6k Kit ELISA, dp70[S6k] Kit ELISA, dp70s6k Kit ELISA, dps6k Kit ELISA, ds6k Kit ELISA, fs(3)07084 Kit ELISA, l(3)07084 Kit ELISA, p70 S6K Kit ELISA, p70/S6K Kit ELISA, p70S6K Kit ELISA, p70[S6 kinase] Kit ELISA, p70[S6K] Kit ELISA, p70[S6k] Kit ELISA, p70[S6kinase] Kit ELISA, p70s6K Kit ELISA, s6k Kit ELISA, s6k11 Kit ELISA, 2610318I15Rik Kit ELISA, 4732464A07Rik Kit ELISA, 70kDa Kit ELISA, AA959758 Kit ELISA, AI256796 Kit ELISA, AI314060 Kit ELISA, p70/85s6k Kit ELISA, p70s6k Kit ELISA, p70s6k-A Kit ELISA, p70-s6k Kit ELISA, ps6k Kit ELISA, rps6kb1 Kit ELISA, rps6kb1-A Kit ELISA, s6K1 Kit ELISA, stk14a Kit ELISA, fc51h01 Kit ELISA, wu:fc51h01 Kit ELISA, zgc:55713 Kit ELISA, ribosomal protein S6 kinase B1 Kit ELISA, Ribosomal protein S6 kinase Kit ELISA, ribosomal protein S6 kinase, polypeptide 1 Kit ELISA, ribosomal protein S6 kinase B1 L homeolog Kit ELISA, ribosomal protein S6 kinase B1 S homeolog Kit ELISA, ribosomal protein S6 kinase b, polypeptide 1b Kit ELISA, RPS6KB1 Kit ELISA, S6k Kit ELISA, Rps6kb1 Kit ELISA, rps6kb1.L Kit ELISA, rps6kb1.S Kit ELISA, rps6kb1b Kit ELISA
ID gène
6198
UniProt
P23443
Pathways
Signalisation PI3K-Akt , Signalisation RTK , AMPK Signaling , Regulation of Cell Size , Skeletal Muscle Fiber Development , Feeding Behaviour , G-protein mediated Events , Smooth Muscle Cell Migration , Interaction of EGFR with phospholipase C-gamma , L'effet Warburg
Vus récemment
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