RAB7A Kit ELISA

N° du produit ABIN6951224

Détail du produit RAB7A Kit ELISA

Antigène: RAB7A (RAB7A, Member RAS Oncogene Family (RAB7A))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Rat RAB7A ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: RAB7A (RAB7A, Member RAS Oncogene Family (RAB7A))

Autre désignation: RAB7A (RAB7A Produits)

Synonymes: RAB5 Kit ELISA, PRO2706 Kit ELISA, RAB7 Kit ELISA, Rab7 Kit ELISA, rab7 Kit ELISA, pro2706 Kit ELISA, RAB7A Kit ELISA, LOC100231734 Kit ELISA, ARABIDOPSIS RAB GTPASE HOMOLOG G2 Kit ELISA, ATRAB7A Kit ELISA, ATRABG2 Kit ELISA, F7D8.20 Kit ELISA, F7D8_20 Kit ELISA, RAB GTPase homolog 7A Kit ELISA, DDBDRAFT_0190210 Kit ELISA, DDBDRAFT_0191507 Kit ELISA, DDB_0190210 Kit ELISA, DDB_0191507 Kit ELISA, Rab7a Kit ELISA, rab-7 Kit ELISA, zgc:64058 Kit ELISA, RAB5A, member RAS oncogene family Kit ELISA, RAB7A, member RAS oncogene family Kit ELISA, RAB7A, member RAS oncogene family L homeolog Kit ELISA, rab7A protein Kit ELISA, RAB GTPase homolog 7A Kit ELISA, Rab GTPase Kit ELISA, ras-related protein rab-7a Kit ELISA, RAB7, member RAS oncogene family Kit ELISA, RAB5A Kit ELISA, RAB7A Kit ELISA, Rab7a Kit ELISA, rab7a.L Kit ELISA, rab7a Kit ELISA, rab7A Kit ELISA, Rab7 Kit ELISA, rab7 Kit ELISA

ID gène: 29448

UniProt: P09527

Pathways: EGFR Signaling Pathway, Maintenance of Protein Location, SARS-CoV-2 Protein Interactome