ROM1 Kit ELISA

N° du produit ABIN6951227

Détail du produit ROM1 Kit ELISA

Antigène: ROM1 (Retinal Outer Segment Membrane Protein 1 (ROM1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human ROSP1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: ROM1 (Retinal Outer Segment Membrane Protein 1 (ROM1))

Autre désignation: ROSP1 (ROM1 Produits)

Synonymes: ROM Kit ELISA, ROSP1 Kit ELISA, RP7 Kit ELISA, TSPAN23 Kit ELISA, ROM1 Kit ELISA, M101156 Kit ELISA, Rgsc1156 Kit ELISA, Rom-1 Kit ELISA, Rosp1 Kit ELISA, Tspan23 Kit ELISA, rds35 Kit ELISA, rom Kit ELISA, rosp1 Kit ELISA, tspan23 Kit ELISA, Rds Kit ELISA, zgc:56548 Kit ELISA, zgc:73336 Kit ELISA, zgc:77401 Kit ELISA, retinal outer segment membrane protein 1 Kit ELISA, rod outer segment membrane protein 1 Kit ELISA, retinal outer segment membrane protein 1 L homeolog Kit ELISA, retinal outer segment membrane protein 1b Kit ELISA, retinal outer segment membrane protein 1a Kit ELISA, ROM1 Kit ELISA, Rom1 Kit ELISA, rom1.L Kit ELISA, rom1b Kit ELISA, rom1a Kit ELISA

ID gène: 6094

UniProt: Q03395