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Syntaxin 7 Kit ELISA

STX7 Reactivité: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951239
  • Antigène Tous les produits Syntaxin 7 (STX7)
    Syntaxin 7 (STX7)
    Reactivité
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Rat STX7 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Tous les produits Syntaxin 7 (STX7)
    Syntaxin 7 (STX7)
    Autre désignation
    STX7 (STX7 Produits)
    Synonymes
    Aval Kit ELISA, Avl Kit ELISA, CG5081 Kit ELISA, Dm Syx7 Kit ELISA, DmSyx7 Kit ELISA, Dmel\\CG5081 Kit ELISA, FCP-C Kit ELISA, SYX7 Kit ELISA, Syx Kit ELISA, avl Kit ELISA, dSyn7 Kit ELISA, syx7 Kit ELISA, syx7/avl Kit ELISA, syntaxin-7 Kit ELISA, F27G20.2 Kit ELISA, SYNTAXIN 24 Kit ELISA, SYP24 Kit ELISA, DDBDRAFT_0187598 Kit ELISA, DDBDRAFT_0231537 Kit ELISA, DDB_0187598 Kit ELISA, DDB_0231537 Kit ELISA, Syntaxin-7 Kit ELISA, STX7 Kit ELISA, AI315064 Kit ELISA, AI317144 Kit ELISA, AW107239 Kit ELISA, Syn7 Kit ELISA, Syntaxin 7 Kit ELISA, syntaxin 7 Kit ELISA, syntaxin Kit ELISA, Syx7 Kit ELISA, STX7 Kit ELISA, ATSYP24 Kit ELISA, syn7A Kit ELISA, stx7 Kit ELISA, Stx7 Kit ELISA
    ID gène
    60466
    UniProt
    O70257
    Pathways
    Synaptic Vesicle Exocytosis
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