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TNFAIP8 Kit ELISA

TNFAIP8 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951264
  • Antigène Tous les produits TNFAIP8
    TNFAIP8 (Tumor Necrosis Factor, alpha-Induced Protein 8 (TNFAIP8))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human TNFAIP8 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Tous les produits TNFAIP8
    TNFAIP8 (Tumor Necrosis Factor, alpha-Induced Protein 8 (TNFAIP8))
    Autre désignation
    TNFAIP8 (TNFAIP8 Produits)
    Synonymes
    MGC55331 Kit ELISA, zgc:55331 Kit ELISA, si:dkey-49m19.6 Kit ELISA, GG2-1 Kit ELISA, MDC-3.13 Kit ELISA, SCC-S2 Kit ELISA, SCCS2 Kit ELISA, AA987150 Kit ELISA, E130304C20Rik Kit ELISA, ENSMUSG00000073567 Kit ELISA, Gg2-1 Kit ELISA, Gm10539 Kit ELISA, Nded Kit ELISA, Ssc-2 Kit ELISA, tumor necrosis factor, alpha-induced protein 8-like 1 Kit ELISA, TNF alpha induced protein 8 S homeolog Kit ELISA, TNF alpha induced protein 8 Kit ELISA, tumor necrosis factor, alpha-induced protein 8 Kit ELISA, tnfaip8l1 Kit ELISA, tnfaip8.S Kit ELISA, TNFAIP8 Kit ELISA, Tnfaip8 Kit ELISA
    ID gène
    25816
    UniProt
    O95379
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