SHPK Kit ELISA

N° du produit ABIN6951296

Détail du produit SHPK Kit ELISA

Antigène: SHPK (Sedoheptulokinase (SHPK))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human SHPK ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: SHPK (Sedoheptulokinase (SHPK))

Autre désignation: SHPK (SHPK Produits)

Synonymes: SHPK Kit ELISA, CARKL Kit ELISA, carkl Kit ELISA, MGC154818 Kit ELISA, SHK Kit ELISA, 4930431K22Rik Kit ELISA, AI194947 Kit ELISA, AW260459 Kit ELISA, Carkl Kit ELISA, sedoheptulokinase Kit ELISA, sedoheptulokinase S homeolog Kit ELISA, Sedoheptulokinase Kit ELISA, SHPK Kit ELISA, shpk.S Kit ELISA, Plabr_2171 Kit ELISA, shpk Kit ELISA, Shpk Kit ELISA

ID gène: 23729

UniProt: Q9UHJ6

Pathways: Cellular Response to Molecule of Bacterial Origin