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LTBR Kit ELISA

LTBR Reactivité: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951313
  • Antigène Voir toutes LTBR Kits ELISA
    LTBR (Lymphotoxin beta Receptor (TNFR Superfamily, Member 3) (LTBR))
    Reactivité
    • 5
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Rat Lymphotoxin beta R ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product LTBR Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Voir toutes LTBR Kits ELISA
    LTBR (Lymphotoxin beta Receptor (TNFR Superfamily, Member 3) (LTBR))
    Autre désignation
    Lymphotoxin beta R (LTBR Produits)
    Synonymes
    AI256028 Kit ELISA, CD18 Kit ELISA, LTbetaR Kit ELISA, Ltar Kit ELISA, TNF-R-III Kit ELISA, TNFCR Kit ELISA, TNFR-RP Kit ELISA, TNFR2-RP Kit ELISA, TNFRrp Kit ELISA, Tnfbr Kit ELISA, Tnfrsf3 Kit ELISA, D12S370 Kit ELISA, LT-BETA-R Kit ELISA, TNFR3 Kit ELISA, TNFRSF3 Kit ELISA, lymphotoxin beta receptor Kit ELISA, lymphotoxin B receptor Kit ELISA, Ltbr Kit ELISA, LTBR Kit ELISA
    ID gène
    297604
    UniProt
    Q5U2S8
    Pathways
    Signalisation NF-kappaB
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