SOD2 Kit ELISA

N° du produit ABIN6951323

Détail du produit SOD2 Kit ELISA

Antigène: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human SOD2 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Autre désignation: SOD2 (SOD2 Produits)

Synonymes: LOC100101896 Kit ELISA, MNSOD Kit ELISA, GB14346 Kit ELISA, sod2 Kit ELISA, MGC88869 Kit ELISA, Sod2 Kit ELISA, CG8905 Kit ELISA, Dmel\\CG8905 Kit ELISA, Mito SOD Kit ELISA, Mn SOD Kit ELISA, Mn-SOD Kit ELISA, Mn-SOD2 Kit ELISA, MnSOD Kit ELISA, MnSODII Kit ELISA, Mn[2+]SOD Kit ELISA, SOD Kit ELISA, SOD-2 Kit ELISA, SOD2 Kit ELISA, Sod-2 Kit ELISA, dSOD2 Kit ELISA, mitSOD2 Kit ELISA, mnSOD Kit ELISA, IPOB Kit ELISA, MVCD6 Kit ELISA, cb463 Kit ELISA, wu:fj33b01 Kit ELISA, zgc:73051 Kit ELISA, MnSOD Kit ELISA, manganese superoxide dismutase Kit ELISA, superoxide dismutase 2, mitochondrial Kit ELISA, superoxide dismutase 2 Kit ELISA, Mn superoxide dismutase Kit ELISA, Superoxide dismutase 2 (Mn) Kit ELISA, superoxide dismutase 2 L homeolog Kit ELISA, BDBG_07234 Kit ELISA, LOC100101896 Kit ELISA, MNSOD Kit ELISA, Sod2 Kit ELISA, sod2 Kit ELISA, LbMnSOD4 Kit ELISA, SOD2 Kit ELISA, LOC100282741 Kit ELISA, SOD-2 Kit ELISA, sod2.L Kit ELISA

ID gène: 6648

UniProt: P04179

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling