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PRMT6 Kit ELISA

PRMT6 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951336
  • Antigène Tous les produits PRMT6
    PRMT6 (Protein Arginine Methyltransferase 6 (PRMT6))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human PRMT6 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Tous les produits PRMT6
    PRMT6 (Protein Arginine Methyltransferase 6 (PRMT6))
    Autre désignation
    PRMT6 (PRMT6 Produits)
    Synonymes
    CG9927 Kit ELISA, DART6 Kit ELISA, Dmel\\CG9927 Kit ELISA, PRMT6 Kit ELISA, HRMT1L6 Kit ELISA, ARABIDOPSIS THALIANA PROTEIN ARGININE METHYLTRANSFERASE 6 Kit ELISA, ATPRMT6 Kit ELISA, protein arginine methyltransferase 6 Kit ELISA, DKFZp459B1431 Kit ELISA, Hrmt1l6 Kit ELISA, hrmt1l6 Kit ELISA, hrmt6 Kit ELISA, im:6908706 Kit ELISA, AW124876 Kit ELISA, BB233495 Kit ELISA, Arginine methyltransferase 6 Kit ELISA, protein arginine methyltransferase 6 Kit ELISA, protein arginine methyltransferase 6 S homeolog Kit ELISA, arginine N-methyltransferase, type I Kit ELISA, protein arginine N-methyltransferase 6 Kit ELISA, Art6 Kit ELISA, PRMT6 Kit ELISA, prmt6.S Kit ELISA, prmt6 Kit ELISA, Prmt6 Kit ELISA
    ID gène
    55170
    UniProt
    Q96LA8
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