PFDN4 Kit ELISA
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- Antigène Voir toutes PFDN4 Kits ELISA
- PFDN4 (Prefoldin Subunit 4 (PFDN4))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Human PFDN4 ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product PFDN4 Kit ELISA
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Date de péremption
- 6 months
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- Antigène Voir toutes PFDN4 Kits ELISA
- PFDN4 (Prefoldin Subunit 4 (PFDN4))
- Autre désignation
- PFDN4 (PFDN4 Produits)
- Synonymes
- DDBDRAFT_0204261 Kit ELISA, DDBDRAFT_0237717 Kit ELISA, DDB_0204261 Kit ELISA, DDB_0237717 Kit ELISA, pfd4 Kit ELISA, C1 Kit ELISA, PFD4 Kit ELISA, 0610025M10Rik Kit ELISA, 1810063B06Rik Kit ELISA, C-1 Kit ELISA, Pfd4 Kit ELISA, si:dkey-222e15.2 Kit ELISA, prefoldin subunit 4 Kit ELISA, prefoldin beta-like domain containing protein Kit ELISA, Prefoldin subunit 4 Kit ELISA, prefoldin 4 Kit ELISA, PFDN4 Kit ELISA, AFUA_1G15240 Kit ELISA, AOR_1_2080174 Kit ELISA, CMU_006340 Kit ELISA, pfdn4 Kit ELISA, pfd4 Kit ELISA, Pfdn4 Kit ELISA
- ID gène
- 5203
- UniProt
- Q9NQP4
- Pathways
- Unfolded Protein Response
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