RRM1 Kit ELISA

N° du produit ABIN6951351

Détail du produit RRM1 Kit ELISA

Antigène: RRM1 (Ribonucleotide Reductase M1 (RRM1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human RRM1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: RRM1 (Ribonucleotide Reductase M1 (RRM1))

Autre désignation: RRM1 (RRM1 Produits)

Synonymes: CG5371 Kit ELISA, DmRNR1 Kit ELISA, Dmel\\CG5371 Kit ELISA, RNR1 Kit ELISA, RnrM1 Kit ELISA, RntL Kit ELISA, l(2)k06709 Kit ELISA, XRRM1 Kit ELISA, R1 Kit ELISA, RIR1 Kit ELISA, RR1 Kit ELISA, CHUNP6866 Kit ELISA, cb396 Kit ELISA, cb838 Kit ELISA, r1 Kit ELISA, sb:cb548 Kit ELISA, wu:fb39b07 Kit ELISA, wu:fi14b02 Kit ELISA, wu:fk95f07 Kit ELISA, ribonucleotide reductase catalytic subunit M1 Kit ELISA, Ribonucleoside diphosphate reductase large subunit Kit ELISA, ribonucleoside-diphosphate reductase large subunit Kit ELISA, ribonucleotide reductase M1 L homeolog Kit ELISA, ribonucleotide reductase M1 Kit ELISA, ribonucleotide reductase M1 polypeptide Kit ELISA, RRM1 Kit ELISA, RnrL Kit ELISA, LOC100472317 Kit ELISA, rrm1 Kit ELISA, rrm1.L Kit ELISA, Rrm1 Kit ELISA

ID gène: 6240

UniProt: P23921

Pathways: Positive Regulation of Endopeptidase Activity