AACS Kit ELISA

N° du produit ABIN6951352

Détail du produit AACS Kit ELISA

Antigène: AACS (Acetoacetyl-CoA Synthetase (AACS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human AACS ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: AACS (Acetoacetyl-CoA Synthetase (AACS))

Autre désignation: AACS (AACS Produits)

Synonymes: sur-5 Kit ELISA, An08g04090 Kit ELISA, AO090001000376 Kit ELISA, ACSF1 Kit ELISA, SUR-5 Kit ELISA, 2210408B16Rik Kit ELISA, SUR5 Kit ELISA, zgc:56105 Kit ELISA, acetoacetyl-CoA synthetase Kit ELISA, bll4282 Kit ELISA, acetoacetyl-coa synthetase Kit ELISA, acetoacetate--CoA ligase Kit ELISA, acetoacetate-CoA ligase Kit ELISA, Acetoacetyl-CoA synthetase Kit ELISA, AACS Kit ELISA, LOC590598 Kit ELISA, aacs Kit ELISA, bll4282 Kit ELISA, Pfl01_3080 Kit ELISA, h16_A2860 Kit ELISA, Mflv_1473 Kit ELISA, ANI_1_594074 Kit ELISA, Pput_2653 Kit ELISA, VIBHAR_RS20435 Kit ELISA, AOR_1_648164 Kit ELISA, CC1G_04522 Kit ELISA, LACBIDRAFT_230952 Kit ELISA, PLES_33261 Kit ELISA, PA1997 Kit ELISA, OB0504 Kit ELISA, CBU_0766 Kit ELISA, acsA2 Kit ELISA, Sde_0006 Kit ELISA, Plav_1154 Kit ELISA, AZC_2243 Kit ELISA, SGR_RS30655 Kit ELISA, Aacs Kit ELISA, sur-5 Kit ELISA

ID gène: 65985

UniProt: Q86V21

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis