PEBP1 Kit ELISA

N° du produit ABIN6951355

Détail du produit PEBP1 Kit ELISA

Antigène: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human PEBP1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Autre désignation: PEBP1 (PEBP1 Produits)

Synonymes: HCNP Kit ELISA, HCNPpp Kit ELISA, PBP Kit ELISA, PEBP Kit ELISA, PEBP-1 Kit ELISA, RKIP Kit ELISA, pbp Kit ELISA, hcnp Kit ELISA, pebp Kit ELISA, rkip Kit ELISA, Pbp Kit ELISA, Pbp1 Kit ELISA, Pbpr Kit ELISA, Rkip Kit ELISA, PEBP1 Kit ELISA, BcDNA:LP12095 Kit ELISA, CG18594 Kit ELISA, Dmel\\CG18594 Kit ELISA, T2 Kit ELISA, zgc:56033 Kit ELISA, zgc:76942 Kit ELISA, phosphatidylethanolamine binding protein 1 Kit ELISA, phosphatidylethanolamine binding protein 1 L homeolog Kit ELISA, phosphatidylethanolamine-binding protein Kit ELISA, phosphatidylethanolamine-binding protein,putative Kit ELISA, Phosphatidylethanolamine-binding protein 1 Kit ELISA, PEBP1 Kit ELISA, pebp1 Kit ELISA, Pebp1 Kit ELISA, pebp1.L Kit ELISA, RR_RS07785 Kit ELISA, PVX_235290 Kit ELISA, PVX_123630 Kit ELISA, PKH_143640 Kit ELISA, EAM_1207 Kit ELISA

ID gène: 5037

UniProt: P30086

Pathways: Feeding Behaviour