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NT5C3 Kit ELISA

NT5C3 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951367
  • Antigène Tous les produits NT5C3
    NT5C3 (5'-Nucleotidase, Cytosolic III (NT5C3))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human NT5C3A ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Tous les produits NT5C3
    NT5C3 (5'-Nucleotidase, Cytosolic III (NT5C3))
    Autre désignation
    NT5C3A (NT5C3 Produits)
    Synonymes
    1600024P05Rik Kit ELISA, 2610206B05Rik Kit ELISA, 3110004A18Rik Kit ELISA, AI842776 Kit ELISA, Nt5c3a Kit ELISA, Nt5y Kit ELISA, PN-1 Kit ELISA, PN-I Kit ELISA, PSN1 Kit ELISA, UMPH Kit ELISA, Umph-1 Kit ELISA, Umph1 Kit ELISA, cN-III Kit ELISA, lupin Kit ELISA, p36 Kit ELISA, fb30h07 Kit ELISA, nt5c3 Kit ELISA, wu:fb30h07 Kit ELISA, zgc:66117 Kit ELISA, NT5C3 Kit ELISA, PYN1 Kit ELISA, P5'N-1 Kit ELISA, P5N-1 Kit ELISA, POMP Kit ELISA, UMPH1 Kit ELISA, hUMP1 Kit ELISA, Nt5c3 Kit ELISA, 5'-nucleotidase, cytosolic III Kit ELISA, 5'-nucleotidase, cytosolic IIIA Kit ELISA, cytosolic 5'-nucleotidase 3A Kit ELISA, Nt5c3 Kit ELISA, nt5c3a Kit ELISA, NT5C3A Kit ELISA, LOC534181 Kit ELISA, Nt5c3a Kit ELISA, nt5c3a.L Kit ELISA, Bm1_56095 Kit ELISA
    ID gène
    51251
    UniProt
    Q9H0P0
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