BRCA1 Kit ELISA
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- Antigène Voir toutes BRCA1 Kits ELISA
- BRCA1 (Breast Cancer 1 (BRCA1))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Human BRCA1 ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product BRCA1 Kit ELISA
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Date de péremption
- 6 months
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- Antigène Voir toutes BRCA1 Kits ELISA
- BRCA1 (Breast Cancer 1 (BRCA1))
- Autre désignation
- BRCA1 (BRCA1 Produits)
- Synonymes
- BRCAI Kit ELISA, BRCC1 Kit ELISA, BROVCA1 Kit ELISA, IRIS Kit ELISA, PNCA4 Kit ELISA, PPP1R53 Kit ELISA, PSCP Kit ELISA, RNF53 Kit ELISA, iris Kit ELISA, pscp Kit ELISA, brcai Kit ELISA, brcc1 Kit ELISA, rnf53 Kit ELISA, brca1 Kit ELISA, BRCA1 Kit ELISA, LOC100224649 Kit ELISA, BRCA1, DNA repair associated Kit ELISA, breast cancer 1 Kit ELISA, breast cancer 1, early onset Kit ELISA, breast cancer 1 L homeolog Kit ELISA, BRCA1 Kit ELISA, brca1 Kit ELISA, LOC755086 Kit ELISA, Brca1 Kit ELISA, brca1.L Kit ELISA
- ID gène
- 672
- UniProt
- P38398
- Pathways
- Cycle Cellulaire, Réparation de l'ADN, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus
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