BCAM Kit ELISA

N° du produit ABIN6951400

Détail du produit BCAM Kit ELISA

Antigène: BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Rat BCAM ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))

Autre désignation: BCAM (BCAM Produits)

Synonymes: cd239 Kit ELISA, msk19 Kit ELISA, MGC81012 Kit ELISA, LU Kit ELISA, prr2 Kit ELISA, zgc:154034 Kit ELISA, 1200005K12Rik Kit ELISA, B-CAM Kit ELISA, Gplu Kit ELISA, Lu Kit ELISA, AU Kit ELISA, CD239 Kit ELISA, MSK19 Kit ELISA, basal cell adhesion molecule (Lutheran blood group) Kit ELISA, basal cell adhesion molecule (Lutheran blood group) L homeolog Kit ELISA, basal cell adhesion molecule Kit ELISA, bcam Kit ELISA, bcam.L Kit ELISA, BCAM Kit ELISA, Bcam Kit ELISA

ID gène: 78958

UniProt: Q9ESS6