CD45 Kit ELISA
PTPRC
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951404
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Antigène
Voir toutes CD45 (PTPRC) Kits ELISA
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CD45 (PTPRC)
(Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
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Reactivité
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Humain
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Méthode de détection
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Colorimetric
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Type de méthode
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Sandwich ELISA
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Application
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ELISA
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Fonction
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Human PTPRC ELISA Kit.
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Type d'échantillon
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Indications d'application
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Optimal working dilution should be determined by the investigator.
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Plaque
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Pre-coated
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Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Date de péremption
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6 months
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Antigène
Voir toutes CD45 (PTPRC) Kits ELISA
-
CD45 (PTPRC)
(Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
-
Autre désignation
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PTPRC (PTPRC Produits)
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Synonymes
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PTPRC Kit ELISA, si:dkeyp-47c5.1 Kit ELISA, B220 Kit ELISA, CD45 Kit ELISA, CD45R Kit ELISA, GP180 Kit ELISA, L-CA Kit ELISA, LCA Kit ELISA, LY5 Kit ELISA, T200 Kit ELISA, Cd45 Kit ELISA, Ly-5 Kit ELISA, Lyt-4 Kit ELISA, loc Kit ELISA, Lca Kit ELISA, RT7 Kit ELISA, cd45 Kit ELISA, protein tyrosine phosphatase, receptor type C Kit ELISA, protein tyrosine phosphatase, receptor type, C Kit ELISA, receptor-type tyrosine-protein phosphatase C Kit ELISA, PTPRC Kit ELISA, ptprc Kit ELISA, LOC100074271 Kit ELISA, Ptprc Kit ELISA
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ID gène
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5788
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UniProt
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P08575
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Pathways
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TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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