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CDKN2D Kit ELISA

CDKN2D Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951426
  • Antigène Tous les produits CDKN2D
    CDKN2D (Cyclin-Dependent Kinase Inhibitor 2D (p19, Inhibits CDK4) (CDKN2D))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human CDKN2D ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Tous les produits CDKN2D
    CDKN2D (Cyclin-Dependent Kinase Inhibitor 2D (p19, Inhibits CDK4) (CDKN2D))
    Autre désignation
    CDKN2D (CDKN2D Produits)
    Synonymes
    ink4d Kit ELISA, p19 Kit ELISA, p19-ink4d Kit ELISA, CDKN2D Kit ELISA, ank2 Kit ELISA, INK4D Kit ELISA, p19-INK4D Kit ELISA, INK4d Kit ELISA, p19INK4d Kit ELISA, cyclin-dependent kinase inhibitor 2D S homeolog Kit ELISA, cyclin dependent kinase inhibitor 2D Kit ELISA, cyclin-dependent kinase inhibitor 2D Kit ELISA, Cyclin-dependent kinase 4 inhibitor D Kit ELISA, cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) Kit ELISA, similar to cyclin-dependent kinase inhibitor 2D Kit ELISA, cdkn2d.S Kit ELISA, CDKN2D Kit ELISA, cdkn2d Kit ELISA, cdn2d Kit ELISA, Cdkn2d Kit ELISA
    ID gène
    1032
    UniProt
    P55273
    Pathways
    Cycle Cellulaire, Sensory Perception of Sound, Mitotic G1-G1/S Phases, Negative Regulation of intrinsic apoptotic Signaling
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