GADD45A Kit ELISA

N° du produit ABIN6951442

Détail du produit GADD45A Kit ELISA

Antigène: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human GADD45A ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Autre désignation: GADD45A (GADD45A Produits)

Synonymes: MGC53491 Kit ELISA, zgc:91795 Kit ELISA, gadd45a Kit ELISA, GADD45A Kit ELISA, ddit1 Kit ELISA, gadd45 Kit ELISA, GADD45 Kit ELISA, AA545191 Kit ELISA, Ddit1 Kit ELISA, DDIT1 Kit ELISA, Gadd45 Kit ELISA, growth arrest and DNA damage inducible alpha L homeolog Kit ELISA, growth arrest and DNA-damage-inducible, alpha, b Kit ELISA, growth arrest and DNA damage inducible alpha Kit ELISA, growth arrest and DNA damage-inducible protein 45 Kit ELISA, growth arrest and DNA-damage-inducible 45 alpha Kit ELISA, growth arrest and DNA-damage-inducible, alpha Kit ELISA, gadd45a.L Kit ELISA, gadd45ab Kit ELISA, GADD45A Kit ELISA, gadd45a Kit ELISA, GADD45 Kit ELISA, Gadd45a Kit ELISA

ID gène: 1647

UniProt: P24522

Pathways: Signalisation p53, Cycle Cellulaire