IKKi/IKKe Kit ELISA
IKBKE
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951456
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Antigène
Voir toutes IKKi/IKKe (IKBKE) Kits ELISA
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IKKi/IKKe (IKBKE)
(Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))
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Reactivité
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Humain
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Méthode de détection
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Colorimetric
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Type de méthode
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Sandwich ELISA
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Application
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ELISA
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Fonction
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Human IKK-E/IKBKE ELISA Kit.
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Type d'échantillon
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Indications d'application
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Optimal working dilution should be determined by the investigator.
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Plaque
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Pre-coated
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Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Date de péremption
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6 months
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Antigène
Voir toutes IKKi/IKKe (IKBKE) Kits ELISA
-
IKKi/IKKe (IKBKE)
(Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))
-
Autre désignation
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IKK-E (IKBKE (IKBKE Produits)
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Synonymes
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zgc:100844 Kit ELISA, IKBKE Kit ELISA, MGC130907 Kit ELISA, IKK-E Kit ELISA, IKK-i Kit ELISA, IKKE Kit ELISA, IKKI Kit ELISA, AW558201 Kit ELISA, IKKepsilon Kit ELISA, Ikki Kit ELISA, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon Kit ELISA, inhibitor of nuclear factor kappa B kinase subunit epsilon Kit ELISA, inhibitor of nuclear factor kappa B kinase subunit epsilon L homeolog Kit ELISA, inhibitor of kappaB kinase epsilon Kit ELISA, IKBKE Kit ELISA, ikbke Kit ELISA, ikbke.L Kit ELISA, Ikbke Kit ELISA
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ID gène
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9641
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UniProt
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Q14164
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Pathways
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Signalisation TLR, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
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