MAPKAP Kinase 3 Kit ELISA

N° du produit ABIN6951469

Détail du produit MAPKAP Kinase 3 Kit ELISA

Antigène: MAPKAP Kinase 3 (MAPKAPK3) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human MAPKAPK3 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: MAPKAP Kinase 3 (MAPKAPK3) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3))

Autre désignation: MAPKAPK3 (MAPKAPK3 Produits)

Synonymes: 3PK Kit ELISA, MAPKAP-K3 Kit ELISA, MAPKAP3 Kit ELISA, MAPKAPK-3 Kit ELISA, MK-3 Kit ELISA, AI874665 Kit ELISA, MK3 Kit ELISA, MapkKapk3 Kit ELISA, MAPKAPK3 Kit ELISA, mapkapk3 Kit ELISA, MGC154360 Kit ELISA, 3pk Kit ELISA, mapkap3 Kit ELISA, zgc:158342 Kit ELISA, mitogen-activated protein kinase-activated protein kinase 3 Kit ELISA, mitogen-activated protein kinase-activated protein kinase 3 S homeolog Kit ELISA, MAPKAPK3 Kit ELISA, Mapkapk3 Kit ELISA, mapkapk3.S Kit ELISA, mapkapk3 Kit ELISA

ID gène: 7867

UniProt: Q16644

Pathways: Signalisation MAPK, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades