Nemo-Like Kinase Kit ELISA
NLK
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951471
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Antigène
Voir toutes Nemo-Like Kinase (NLK) Kits ELISA
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Nemo-Like Kinase (NLK)
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Reactivité
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Humain
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Méthode de détection
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Colorimetric
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Type de méthode
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Sandwich ELISA
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Application
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ELISA
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Fonction
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Human NLK ELISA Kit.
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Type d'échantillon
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Indications d'application
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Optimal working dilution should be determined by the investigator.
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Plaque
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Pre-coated
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Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Date de péremption
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6 months
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Antigène
Voir toutes Nemo-Like Kinase (NLK) Kits ELISA
-
Nemo-Like Kinase (NLK)
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Autre désignation
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NLK (NLK Produits)
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Synonymes
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Amf Kit ELISA, Gpi1 Kit ELISA, AI194375 Kit ELISA, nlk Kit ELISA, nlkb Kit ELISA, zgc:111875 Kit ELISA, Xnlk Kit ELISA, nlk1 Kit ELISA, nlk2 Kit ELISA, NLK Kit ELISA, nemo-like Kit ELISA, RGD1561602 Kit ELISA, glucose-6-phosphate isomerase Kit ELISA, nemo like kinase Kit ELISA, nemo-like kinase, type 1 Kit ELISA, nemo-like kinase L homeolog Kit ELISA, Nemo-Like kinase Kit ELISA, nemo-like kinase Kit ELISA, Gpi Kit ELISA, NLK Kit ELISA, Nlk Kit ELISA, nlk1 Kit ELISA, nlk.L Kit ELISA, nlk Kit ELISA, nemo-like Kit ELISA
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ID gène
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51701
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UniProt
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Q9UBE8
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Pathways
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Ubiquitin Proteasome Pathway
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