m1ip1 Kit ELISA

N° du produit ABIN6951500

Détail du produit m1ip1 Kit ELISA

Antigène: m1ip1 (Mid1-Interacting Protein 1 (m1ip1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human MID1IP1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: m1ip1 (Mid1-Interacting Protein 1 (m1ip1))

Autre désignation: MID1IP1 (m1ip1 Produits)

Synonymes: MIG12 Kit ELISA, TB10 Kit ELISA, G12-like Kit ELISA, S14R Kit ELISA, STRAIT11499 Kit ELISA, THRSPL Kit ELISA, 3110038L01Rik Kit ELISA, Mig12 Kit ELISA, slap Kit ELISA, mig12 Kit ELISA, thrspl Kit ELISA, MGC80850 Kit ELISA, zgc:73223 Kit ELISA, g12 Kit ELISA, wu:fj32f05 Kit ELISA, thymosin beta 10 Kit ELISA, MID1 interacting protein 1 Kit ELISA, Mid1-interacting protein 1 Kit ELISA, Mid1 interacting protein 1 (gastrulation specific G12-like (zebrafish)) Kit ELISA, MID1 interacting protein 1 S homeolog Kit ELISA, MID1 interacting protein 1b Kit ELISA, MID1 interacting protein 1a Kit ELISA, TMSB10 Kit ELISA, MID1IP1 Kit ELISA, m1ip1 Kit ELISA, Mid1ip1 Kit ELISA, mid1ip1.S Kit ELISA, mid1ip1b Kit ELISA, mid1ip1a Kit ELISA

ID gène: 58526

UniProt: Q9NPA3