GPER Kit ELISA

N° du produit ABIN6951509

Détail du produit GPER Kit ELISA

Antigène: GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human GPER/GPR30 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

Autre désignation: GPER (GPR30 (GPER Produits)

Synonymes: CEPR Kit ELISA, CMKRL2 Kit ELISA, DRY12 Kit ELISA, FEG-1 Kit ELISA, GPCR-Br Kit ELISA, GPER Kit ELISA, GPR30 Kit ELISA, LERGU Kit ELISA, LERGU2 Kit ELISA, LyGPR Kit ELISA, 6330420K13Rik Kit ELISA, Ceprl Kit ELISA, Gper Kit ELISA, Gpr30 Kit ELISA, GPR41 Kit ELISA, GPCR30 Kit ELISA, G protein-coupled estrogen receptor 1 Kit ELISA, GPER1 Kit ELISA, GPER Kit ELISA, Gper1 Kit ELISA

ID gène: 2852

UniProt: Q99527

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process