14-3-3 theta Kit ELISA

N° du produit ABIN6951519

Détail du produit 14-3-3 theta Kit ELISA

Antigène: 14-3-3 theta (YWHAQ)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human 14-3-3 theta ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: 14-3-3 theta (YWHAQ)

Autre désignation: 14-3-3 theta (YWHAQ Produits)

Synonymes: 14-3-3 Kit ELISA, 14-3-3t Kit ELISA, 1c5 Kit ELISA, hs1 Kit ELISA, 1C5 Kit ELISA, HS1 Kit ELISA, 2700028P07Rik Kit ELISA, AA409740 Kit ELISA, AU021156 Kit ELISA, R74690 Kit ELISA, ywhaq Kit ELISA, ywhaq2 Kit ELISA, wu:fa91b01 Kit ELISA, wu:fb02e12 Kit ELISA, wu:fb36f06 Kit ELISA, wu:fk86h02 Kit ELISA, ywhaq1 Kit ELISA, ywhaz Kit ELISA, zgc:55499 Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta S homeolog Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide a Kit ELISA, 14-3-3 protein theta Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide b Kit ELISA, YWHAQ Kit ELISA, ywhaq Kit ELISA, Ywhaq Kit ELISA, ywhaq.S Kit ELISA, ywhaqa Kit ELISA, ywhaqb Kit ELISA

ID gène: 10971

UniProt: P27348

Pathways: Apoptose, Myometrial Relaxation and Contraction