ACOX1 Kit ELISA

N° du produit ABIN6951524

Détail du produit ACOX1 Kit ELISA

Antigène: ACOX1 (Acyl-CoA Oxidase 1, Palmitoyl (ACOX1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human ACOX1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: ACOX1 (Acyl-CoA Oxidase 1, Palmitoyl (ACOX1))

Autre désignation: ACOX1 (ACOX1 Produits)

Synonymes: zgc:92584 Kit ELISA, wu:fb59h12 Kit ELISA, zgc:114033 Kit ELISA, aco Kit ELISA, acox Kit ELISA, ACOX Kit ELISA, PALMCOX Kit ELISA, SCOX Kit ELISA, RATACOA1 Kit ELISA, AOX Kit ELISA, Acox Kit ELISA, D130055E20Rik Kit ELISA, Paox Kit ELISA, PCOX1 Kit ELISA, acyl-CoA oxidase 1 Kit ELISA, acyl-CoA oxidase 1, palmitoyl Kit ELISA, acyl-CoA oxidase 1, palmitoyl L homeolog Kit ELISA, acyl-Coenzyme A oxidase 1, palmitoyl Kit ELISA, ACOX1 Kit ELISA, acox1 Kit ELISA, acox1.L Kit ELISA, Acox1 Kit ELISA

ID gène: 51

UniProt: Q15067

Pathways: Regulation of Lipid Metabolism by PPARalpha, Monocarboxylic Acid Catabolic Process