PHAP1 Kit ELISA

N° du produit ABIN6951526

Détail du produit PHAP1 Kit ELISA

Antigène: PHAP1 (ANP32A) (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human ANP32A ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: PHAP1 (ANP32A) (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A))

Autre désignation: ANP32A (ANP32A Produits)

Synonymes: C15orf1 Kit ELISA, HPPCn Kit ELISA, I1PP2A Kit ELISA, LANP Kit ELISA, MAPM Kit ELISA, PHAP1 Kit ELISA, PHAPI Kit ELISA, PP32 Kit ELISA, Anp32 Kit ELISA, W91701 Kit ELISA, pp32 Kit ELISA, Anp32a Kit ELISA, CG5784 Kit ELISA, CT18148 Kit ELISA, CT42180 Kit ELISA, Dmel\\CG5784 Kit ELISA, anon-EST:fe3A2 Kit ELISA, wu:fj36e08 Kit ELISA, zgc:55516 Kit ELISA, ANP32D Kit ELISA, acidic nuclear phosphoprotein 32 family member A Kit ELISA, acidic (leucine-rich) nuclear phosphoprotein 32 family, member A Kit ELISA, CG5784 gene product from transcript CG5784-RB Kit ELISA, acidic nuclear phosphoprotein 32 family member A L homeolog Kit ELISA, ANP32A Kit ELISA, Anp32a Kit ELISA, Mapmodulin Kit ELISA, anp32a Kit ELISA, anp32a.L Kit ELISA

ID gène: 8125

UniProt: P39687