ATG4B Kit ELISA

N° du produit ABIN6951529

Détail du produit ATG4B Kit ELISA

Antigène: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human ATG4B ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Autre désignation: ATG4B (ATG4B Produits)

Synonymes: apg4b Kit ELISA, APG4B Kit ELISA, AUTL1 Kit ELISA, 2510009N07Rik Kit ELISA, AW048066 Kit ELISA, Apg4b Kit ELISA, Atg4bl Kit ELISA, Autl1 Kit ELISA, Aut2b2 Kit ELISA, autophagy related 4B cysteine peptidase Kit ELISA, autophagy-related 4b Kit ELISA, Peptidase family C54 protein Kit ELISA, cysteine protease ATG4B Kit ELISA, ATG4 autophagy related 4 homolog B (S. cerevisiae) Kit ELISA, autophagy related 4B, cysteine peptidase Kit ELISA, autophagy related 4B, cysteine peptidase L homeolog Kit ELISA, ATG4B Kit ELISA, Atg4b Kit ELISA, AT3G59950 Kit ELISA, Tsp_08582 Kit ELISA, Tsp_11599 Kit ELISA, atg4b Kit ELISA, atg4b.L Kit ELISA

ID gène: 23192

UniProt: Q9Y4P1

Pathways: Autophagy