ERBB4 Kit ELISA
ERBB4
Reactivité: Rat
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951544
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Antigène
Voir toutes ERBB4 Kits ELISA
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ERBB4
(V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))
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Reactivité
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Rat
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Méthode de détection
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Colorimetric
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Type de méthode
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Sandwich ELISA
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Application
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ELISA
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Fonction
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Rat ErbB4 ELISA Kit.
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Type d'échantillon
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Indications d'application
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Optimal working dilution should be determined by the investigator.
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Plaque
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Pre-coated
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Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Date de péremption
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6 months
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Antigène
Voir toutes ERBB4 Kits ELISA
-
ERBB4
(V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))
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Autre désignation
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ErbB4 (ERBB4 Produits)
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Synonymes
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erbb4 Kit ELISA, si:ch211-227n20.1 Kit ELISA, Her4 Kit ELISA, c-erbB-4 Kit ELISA, HER4 Kit ELISA, p180erbB4 Kit ELISA, erb-b2 receptor tyrosine kinase 4 Kit ELISA, erb-b2 receptor tyrosine kinase 4a Kit ELISA, v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) Kit ELISA, erb-b2 receptor tyrosine kinase 4 L homeolog Kit ELISA, receptor tyrosine-protein kinase ErbB-4 Kit ELISA, ERBB4 Kit ELISA, erbb4a Kit ELISA, erbb4 Kit ELISA, erbb4.L Kit ELISA, Tsp_13582 Kit ELISA, Erbb4 Kit ELISA
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ID gène
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59323
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UniProt
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Q62956
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Pathways
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Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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