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ERBB4 Kit ELISA

ERBB4 Reactivité: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951544
  • Antigène Voir toutes ERBB4 Kits ELISA
    ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))
    Reactivité
    • 6
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Rat ErbB4 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Voir toutes ERBB4 Kits ELISA
    ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))
    Autre désignation
    ErbB4 (ERBB4 Produits)
    Synonymes
    erbb4 Kit ELISA, si:ch211-227n20.1 Kit ELISA, Her4 Kit ELISA, c-erbB-4 Kit ELISA, HER4 Kit ELISA, p180erbB4 Kit ELISA, erb-b2 receptor tyrosine kinase 4 Kit ELISA, erb-b2 receptor tyrosine kinase 4a Kit ELISA, v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) Kit ELISA, erb-b2 receptor tyrosine kinase 4 L homeolog Kit ELISA, receptor tyrosine-protein kinase ErbB-4 Kit ELISA, ERBB4 Kit ELISA, erbb4a Kit ELISA, erbb4 Kit ELISA, erbb4.L Kit ELISA, Tsp_13582 Kit ELISA, Erbb4 Kit ELISA
    ID gène
    59323
    UniProt
    Q62956
    Pathways
    Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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