GNB2L1 Kit ELISA
GNB2L1
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951608
-
-
Antigène
Tous les produits GNB2L1
-
GNB2L1
(Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1))
-
Reactivité
-
Humain
-
Méthode de détection
-
Colorimetric
-
Type de méthode
-
Sandwich ELISA
-
Application
-
ELISA
-
Fonction
-
Human GNB2L1/RACK1 ELISA Kit.
-
Type d'échantillon
-
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
Analytical Method
-
Quantitative
-
Attributs du produit
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
Ingrédients
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
Matériel non inclus
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
Indications d'application
-
Optimal working dilution should be determined by the investigator.
-
Plaque
-
Pre-coated
-
Protocole
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
-
Restrictions
-
For Research Use only
-
-
Date de péremption
-
6 months
-
-
Antigène
Tous les produits GNB2L1
-
GNB2L1
(Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1))
-
Autre désignation
-
GNB2L1 (RACK1 (GNB2L1 Produits)
-
Synonymes
-
MGC53289 Kit ELISA, GNB2L1 Kit ELISA, AL033335 Kit ELISA, GB-like Kit ELISA, Gnb2-rs1 Kit ELISA, Rack1 Kit ELISA, p205 Kit ELISA, RACK1 Kit ELISA, H12.3 Kit ELISA, HLC-7 Kit ELISA, PIG21 Kit ELISA, rack1 Kit ELISA, wu:fb80d08 Kit ELISA, wu:fk65d12 Kit ELISA, gnb2-rs1 Kit ELISA, h12.3 Kit ELISA, hlc-7 Kit ELISA, pig21 Kit ELISA, C12-3 Kit ELISA, c12.3 Kit ELISA, guanine nucleotide binding protein, beta 2, related sequence 1 Kit ELISA, receptor for activated C kinase 1 Kit ELISA, guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 Kit ELISA, receptor for activated C kinase 1 L homeolog Kit ELISA, Guanine nucleotide-binding protein subunit beta-2-like 1 Kit ELISA, gnb2l1 Kit ELISA, RACK1 Kit ELISA, Rack1 Kit ELISA, rack1.L Kit ELISA, rack-1 Kit ELISA
-
ID gène
-
10399
-
UniProt
-
P63244
-
Pathways
-
cAMP Metabolic Process, Positive Regulation of Endopeptidase Activity
-