MDH1 Kit ELISA

N° du produit ABIN6951670

Détail du produit MDH1 Kit ELISA

Antigène: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Rat MDH1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Autre désignation: MDH1 (MDH1 Produits)

Synonymes: MDH-s Kit ELISA, MDHA Kit ELISA, MGC:1375 Kit ELISA, MOR2 Kit ELISA, ODS1 Kit ELISA, B230377B03Rik Kit ELISA, D17921 Kit ELISA, Mor-2 Kit ELISA, Mor2 Kit ELISA, MDL1 Kit ELISA, Mdhl Kit ELISA, MDH Kit ELISA, mdh-s Kit ELISA, mdha Kit ELISA, mor2 Kit ELISA, MDH-1 Kit ELISA, Mdh Kit ELISA, Mdh-1 Kit ELISA, Mdh2 Kit ELISA, MdhD Kit ELISA, cMdh Kit ELISA, sMdh Kit ELISA, malate dehydrogenase 1 Kit ELISA, malic enzyme 2 Kit ELISA, malate dehydrogenase 1, NAD (soluble) Kit ELISA, malate dehydrogenase 1 S homeolog Kit ELISA, malate dehydrogenase, cytoplasmic Kit ELISA, Malate dehydrogenase 1 Kit ELISA, MDH1 Kit ELISA, ME2 Kit ELISA, Mdh1 Kit ELISA, mdh1.S Kit ELISA, LOC100280767 Kit ELISA

ID gène: 24551

UniProt: O88989