PCSK1 Kit ELISA
PCSK1
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951681
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Antigène
Voir toutes PCSK1 Kits ELISA
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PCSK1
(Proprotein Convertase Subtilisin/kexin Type 1 (PCSK1))
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Reactivité
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Humain
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Méthode de détection
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Colorimetric
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Type de méthode
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Sandwich ELISA
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Application
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ELISA
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Fonction
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Human PCSK1 ELISA Kit.
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Type d'échantillon
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Top Product
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Discover our top product PCSK1 Kit ELISA
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Indications d'application
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Optimal working dilution should be determined by the investigator.
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Plaque
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Pre-coated
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Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Date de péremption
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6 months
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Antigène
Voir toutes PCSK1 Kits ELISA
-
PCSK1
(Proprotein Convertase Subtilisin/kexin Type 1 (PCSK1))
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Autre désignation
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PCSK1 (PCSK1 Produits)
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Synonymes
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BMIQ12 Kit ELISA, NEC1 Kit ELISA, PC1 Kit ELISA, PC3 Kit ELISA, SPC3 Kit ELISA, Nec-1 Kit ELISA, Nec1 Kit ELISA, Phpp-1 Kit ELISA, BDP Kit ELISA, PC1/3 Kit ELISA, proprotein convertase subtilisin/kexin type 1 Kit ELISA, prohormone convertase 1 Kit ELISA, PCSK1 Kit ELISA, Pcsk1 Kit ELISA, pcsk1 Kit ELISA, PC1B Kit ELISA
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ID gène
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5122
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UniProt
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P29120
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Pathways
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Peptide Hormone Metabolism
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