PHGDH Kit ELISA

N° du produit ABIN6951683

Détail du produit PHGDH Kit ELISA

Antigène: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human PHGDH ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Autre désignation: PHGDH (PHGDH Produits)

Synonymes: fb38f06 Kit ELISA, zgc:65956 Kit ELISA, wu:fb38f06 Kit ELISA, embryo sac development arrest 9 Kit ELISA, F10M10.7 Kit ELISA, ECK2909 Kit ELISA, JW2880 Kit ELISA, 3-PGDH Kit ELISA, 3PGDH Kit ELISA, 4930479N23 Kit ELISA, A10 Kit ELISA, PGAD Kit ELISA, PGD Kit ELISA, PGDH Kit ELISA, SERA Kit ELISA, PDG Kit ELISA, phosphoglycerate dehydrogenase Kit ELISA, D-3-phosphoglycerate dehydrogenase Kit ELISA, 3-phosphoglycerate dehydrogenase Kit ELISA, phgdh Kit ELISA, EDA9 Kit ELISA, serA Kit ELISA, Sthe_2284 Kit ELISA, Dacet_1260 Kit ELISA, Mrub_0173 Kit ELISA, MMAH_RS07650 Kit ELISA, Arnit_0487 Kit ELISA, Ndas_0174 Kit ELISA, Mesil_2386 Kit ELISA, Slip_0010 Kit ELISA, Trad_0302 Kit ELISA, Acear_0027 Kit ELISA, Mahau_0665 Kit ELISA, MZHIL_RS05165 Kit ELISA, Mesop_1331 Kit ELISA, Thein_0855 Kit ELISA, Phgdh Kit ELISA, PHGDH Kit ELISA

ID gène: 26227

UniProt: O43175

Pathways: Metabolism of Steroid Hormones and Vitamin D, L'effet Warburg