PPCDC Kit ELISA
PPCDC
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951684
-
-
Antigène
Voir toutes PPCDC Kits ELISA
-
PPCDC
(phosphopantothenoylcysteine Decarboxylase (PPCDC))
-
Reactivité
-
Humain
-
Méthode de détection
-
Colorimetric
-
Type de méthode
-
Sandwich ELISA
-
Application
-
ELISA
-
Fonction
-
Human PPCDC ELISA Kit.
-
Type d'échantillon
-
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
Analytical Method
-
Quantitative
-
Attributs du produit
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
Ingrédients
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
Matériel non inclus
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
-
Indications d'application
-
Optimal working dilution should be determined by the investigator.
-
Plaque
-
Pre-coated
-
Protocole
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
-
Restrictions
-
For Research Use only
-
-
Date de péremption
-
6 months
-
-
Antigène
Voir toutes PPCDC Kits ELISA
-
PPCDC
(phosphopantothenoylcysteine Decarboxylase (PPCDC))
-
Autre désignation
-
PPCDC (PPCDC Produits)
-
Synonymes
-
MDS018 Kit ELISA, 1810057I13Rik Kit ELISA, 8430432M10Rik Kit ELISA, RGD1306267 Kit ELISA, phosphopantothenoylcysteine decarboxylase Kit ELISA, PPCDC Kit ELISA, Ppcdc Kit ELISA
-
ID gène
-
60490
-
UniProt
-
Q96CD2
-
Pathways
-
Ribonucleoside Biosynthetic Process
-