RUVBL1 Kit ELISA

N° du produit ABIN6951691

Détail du produit RUVBL1 Kit ELISA

Antigène: RUVBL1 (RuvB-Like 1 (E. Coli) (RUVBL1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human RUVBL1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: RUVBL1 (RuvB-Like 1 (E. Coli) (RUVBL1))

Autre désignation: RUVBL1 (RUVBL1 Produits)

Synonymes: rvb1 Kit ELISA, ecp54 Kit ELISA, tip49 Kit ELISA, nmp238 Kit ELISA, pontin Kit ELISA, tip49a Kit ELISA, xpontin Kit ELISA, pontin52 Kit ELISA, TIP49 Kit ELISA, wu:fd18e04 Kit ELISA, zpontin Kit ELISA, ECP54 Kit ELISA, INO80H Kit ELISA, NMP238 Kit ELISA, PONTIN Kit ELISA, Pontin52 Kit ELISA, RVB1 Kit ELISA, TIH1 Kit ELISA, TIP49A Kit ELISA, Tip49a Kit ELISA, 2510009G06Rik Kit ELISA, RuvB like AAA ATPase 1 Kit ELISA, RuvB-like 1 (E. coli) Kit ELISA, RuvB-like 1 Kit ELISA, RuvB like AAA ATPase 1 S homeolog Kit ELISA, RuvB-like AAA ATPase 1 Kit ELISA, RuvB-like protein 1 Kit ELISA, RUVBL1 Kit ELISA, ruvbl1 Kit ELISA, PVX_123295 Kit ELISA, Bm1_15605 Kit ELISA, ruvb1 Kit ELISA, ruvbl1.S Kit ELISA, Ruvbl1 Kit ELISA

ID gène: 8607

UniProt: Q9Y265

Pathways: Telomere Maintenance