NCKIPSD Kit ELISA

N° du produit ABIN6951696

Détail du produit NCKIPSD Kit ELISA

Antigène: NCKIPSD (NCK Interacting Protein with SH3 Domain (NCKIPSD))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human NCKIPSD ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: NCKIPSD (NCK Interacting Protein with SH3 Domain (NCKIPSD))

Autre désignation: NCKIPSD (NCKIPSD Produits)

Synonymes: AF3P21 Kit ELISA, DIP Kit ELISA, DIP1 Kit ELISA, ORF1 Kit ELISA, SPIN90 Kit ELISA, VIP54 Kit ELISA, WASLBP Kit ELISA, WISH Kit ELISA, Wasbp Kit ELISA, SH3 adapter protein SPIN90 Kit ELISA, NCK interacting protein with SH3 domain Kit ELISA, CpipJ_CPIJ019280 Kit ELISA, NCKIPSD Kit ELISA, Nckipsd Kit ELISA

ID gène: 51517

UniProt: Q9NZQ3

Pathways: Protein targeting to Nucleus