SRPK1 Kit ELISA

N° du produit ABIN6951698

Détail du produit SRPK1 Kit ELISA

Antigène: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human SRPK1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Autre désignation: SRPK1 (SRPK1 Produits)

Synonymes: SFRSK1 Kit ELISA, AU017960 Kit ELISA, BEST:CK01209 Kit ELISA, CG11489 Kit ELISA, CG9085 Kit ELISA, CK01209 Kit ELISA, Dmel\\CG11489 Kit ELISA, SRPK Kit ELISA, SRPK1 Kit ELISA, Srpk79D Kit ELISA, atc Kit ELISA, srpk1 Kit ELISA, zgc:63789 Kit ELISA, wu:fc06h03 Kit ELISA, zgc:158800 Kit ELISA, sfrsk1 Kit ELISA, SRSF protein kinase 1 Kit ELISA, serine/arginine-rich protein specific kinase 1 Kit ELISA, Serine-arginine protein kinase at 79D Kit ELISA, SRSF protein kinase 1a Kit ELISA, SRSF protein kinase 1b Kit ELISA, SRSF protein kinase 1 S homeolog Kit ELISA, SRPK1 Kit ELISA, Srpk1 Kit ELISA, Srpk79D Kit ELISA, srpk1a Kit ELISA, srpk1b Kit ELISA, srpk1.S Kit ELISA

ID gène: 6732

UniProt: Q96SB4

Pathways: Toll-Like Receptors Cascades