GFPT1 Kit ELISA

N° du produit ABIN6951707

Détail du produit GFPT1 Kit ELISA

Antigène: GFPT1 (Glutamine--Fructose-6-Phosphate Transaminase 1 (GFPT1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human GFPT1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: GFPT1 (Glutamine--Fructose-6-Phosphate Transaminase 1 (GFPT1))

Autre désignation: GFPT1 (GFPT1 Produits)

Synonymes: gfat Kit ELISA, 2810423A18Rik Kit ELISA, AI324119 Kit ELISA, AI449986 Kit ELISA, GFA Kit ELISA, GFAT Kit ELISA, GFAT1 Kit ELISA, GFAT1m Kit ELISA, Gfpt Kit ELISA, CMSTA1 Kit ELISA, GFAT 1 Kit ELISA, GFPT Kit ELISA, GFPT1L Kit ELISA, MSLG Kit ELISA, glutamine--fructose-6-phosphate transaminase 1 Kit ELISA, glutamine--fructose-6-phosphate transaminase 1 S homeolog Kit ELISA, glutamine fructose-6-phosphate transaminase 1 Kit ELISA, GFPT1 Kit ELISA, gfpt1.S Kit ELISA, Gfpt1 Kit ELISA

ID gène: 2673

UniProt: Q06210

Pathways: ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process