RPRD1B Kit ELISA

N° du produit ABIN6951711

Détail du produit RPRD1B Kit ELISA

Antigène: RPRD1B (Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human RPRD1B ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: RPRD1B (Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B))

Autre désignation: RPRD1B (RPRD1B Produits)

Synonymes: fb23h08 Kit ELISA, zgc:55687 Kit ELISA, wu:fb23h08 Kit ELISA, rprd1ba Kit ELISA, MGC130705 Kit ELISA, C13H20ORF77 Kit ELISA, C20orf77 Kit ELISA, CREPT Kit ELISA, NET60 Kit ELISA, dJ1057B20.2 Kit ELISA, 2610304G08Rik Kit ELISA, 2810446G03Rik Kit ELISA, Crept Kit ELISA, RGD1304782 Kit ELISA, regulation of nuclear pre-mRNA domain containing 1B Kit ELISA, regulation of nuclear pre-mRNA domain containing 1B L homeolog Kit ELISA, rprd1b Kit ELISA, rprd1b.L Kit ELISA, RPRD1B Kit ELISA, Rprd1b Kit ELISA

ID gène: 58490

UniProt: Q9NQG5