SEMA3F Kit ELISA
SEMA3F
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951718
-
-
Antigène
Voir toutes SEMA3F Kits ELISA
-
SEMA3F
(Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3F (SEMA3F))
-
Reactivité
-
Souris
-
Méthode de détection
-
Colorimetric
-
Type de méthode
-
Sandwich ELISA
-
Application
-
ELISA
-
Fonction
-
Mouse SEMA3F ELISA Kit.
-
Type d'échantillon
-
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
Analytical Method
-
Quantitative
-
Attributs du produit
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
Ingrédients
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
Matériel non inclus
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
Top Product
-
Discover our top product SEMA3F Kit ELISA
-
-
-
Indications d'application
-
Optimal working dilution should be determined by the investigator.
-
Plaque
-
Pre-coated
-
Protocole
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
-
Restrictions
-
For Research Use only
-
-
Date de péremption
-
6 months
-
-
Antigène
Voir toutes SEMA3F Kits ELISA
-
SEMA3F
(Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3F (SEMA3F))
-
Autre désignation
-
SEMA3F (SEMA3F Produits)
-
Synonymes
-
Sema4 Kit ELISA, Semak Kit ELISA, SemaIV Kit ELISA, semaphorin-3F Kit ELISA, sema4 Kit ELISA, semak Kit ELISA, sema-iv Kit ELISA, SEMA3F Kit ELISA, SEMA-IV Kit ELISA, SEMA4 Kit ELISA, SEMAK Kit ELISA, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F Kit ELISA, semaphorin 3F Kit ELISA, semaphorin 3F L homeolog Kit ELISA, Sema3f Kit ELISA, SEMA3F Kit ELISA, sema3f Kit ELISA, sema3f.L Kit ELISA
-
ID gène
-
20350
-
UniProt
-
O88632
-
Pathways
-
Regulation of Cell Size
-