ATG10 Kit ELISA

N° du produit ABIN6951722

Détail du produit ATG10 Kit ELISA

Antigène: ATG10 (Autophagy Related 10 (ATG10))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human ATG10 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: ATG10 (Autophagy Related 10 (ATG10))

Autre désignation: ATG10 (ATG10 Produits)

Synonymes: APG10 Kit ELISA, APG10L Kit ELISA, pp12616 Kit ELISA, Atg10 Kit ELISA, ATG10 Kit ELISA, MGC123341 Kit ELISA, im:7153729 Kit ELISA, zgc:123341 Kit ELISA, ATATG10 Kit ELISA, autophagy 10 Kit ELISA, DDBDRAFT_0190076 Kit ELISA, DDBDRAFT_0252608 Kit ELISA, DDB_0190076 Kit ELISA, DDB_0252608 Kit ELISA, 5330424L23Rik Kit ELISA, 5430428K15Rik Kit ELISA, AI852123 Kit ELISA, Agp10 Kit ELISA, Apg10l Kit ELISA, Apg10p Kit ELISA, Atg10l Kit ELISA, autophagy related 10 Kit ELISA, autophagy-related 10 Kit ELISA, AuTophaGy (yeast Atg homolog) Kit ELISA, ATG10 autophagy related 10 homolog (S. cerevisiae) Kit ELISA, autophagocytosis-associated family protein Kit ELISA, E2-like conjugating enzyme Kit ELISA, hypothetical protein Kit ELISA, autophagy protein 10 Kit ELISA, autophagy-related protein 10 Kit ELISA, ATG10 Kit ELISA, Atg10 Kit ELISA, atg-10 Kit ELISA, atg10 Kit ELISA, CAALFM_C302930WA Kit ELISA, APG10 Kit ELISA

ID gène: 83734

UniProt: Q9H0Y0

Pathways: ER-Nucleus Signaling, Autophagy