FHIT Kit ELISA

N° du produit ABIN6951726

Détail du produit FHIT Kit ELISA

Antigène: FHIT (Fragile Histidine Triad (FHIT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human FHIT ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: FHIT (Fragile Histidine Triad (FHIT))

Autre désignation: FHIT (FHIT Produits)

Synonymes: AP3Aase Kit ELISA, FRA3B Kit ELISA, FHIT Kit ELISA, zgc:73176 Kit ELISA, AW045638 Kit ELISA, Fra14A2 Kit ELISA, LOC100223655 Kit ELISA, fragile histidine triad Kit ELISA, fragile histidine triad gene Kit ELISA, fragile histidine triad protein Kit ELISA, fragile histidine triad L homeolog Kit ELISA, FHIT Kit ELISA, Fhit Kit ELISA, fhit Kit ELISA, PY07476 Kit ELISA, fhit.L Kit ELISA

ID gène: 2272

UniProt: P49789